High Throughput Screening of Isomers

TECHNOLOGY OPPORTUNITY

Florida International University (FIU) is pursuing a business partner interested in developing and commercializing a Fast and Effective Screening Workflow for the Separation, Identification, and Quantification of Isomers based on differences in their tridimensional structure under different substrates and physiological conditions.

Natural products play important roles in drug development. In Particular, ribosomally synthesized and post-translationally modified peptides (RiPPs) present a broad structural diversity, typically restricting conformational flexibility to allow better target recognition and to increase chemical, physical and proteolytic stability augmenting chemical functionality. For example, lasso peptides are a structural class of RiPPs exhibiting enzyme inhibitory, receptor antagonistic, antimicrobial or antiviral properties.  The extraordinary mechanically interlocked topology of lasso peptides, together with their panel of biological activities makes them a promising scaffold for next generation drug design. However, the discovery and search for new lasso peptides as potential drug candidates requires high throughput analytical tools capable of differentiating them from their unthreaded branched-cyclic topoisomers.

FIU inventors have developed methods for the identification and isolation of isomers, particularly, of RiPPs and other biomolecules. These methods allow for the separation and isolation of topoisomers, such as lasso peptides from the corresponding branched cyclic peptide isomers, epimers of peptides containing D amino acids, RiPPs positional isomers based on differences in the tridimensional structure under different substrates and physiological conditions. The methods comprise subjecting a sample to a step of ionization comprising a step of metalation, prior to a step of ion mobility spectrometry (IMS) followed by a step of mass spectrometry (MS).

APPLICATIONS
  • Separation, identification and quantification of isomers of RiPPs for drug design and development
ADVANTAGES
  • Permits the direct analysis of complex biological samples and the identification of isomers of RiPPs
  • Separates isomers of RiPPs having a molecular weight between 400 Da to 1000 kDa
QUESTIONS ABOUT THIS TECHNOLOGY

Call Anne-Laure “Anlo” Schmitt-Olivier at 305-348-5948 or fill out the quick contact form below.