Novel Azulenyl Nitrone Spin Trapping Agents

Tagging free radicals via characteristically colored and highly visible spin adducts

TECHNOLOGY OPPORTUNITY

Florida International University is pursuing a commercial partner interested in developing Novel Spin Trapping Agents. Spin trapping agents have been widely used for freed radical research and therapeutic applications. The superoxide radical is considered to play important roles in physiological processes as well as in the genesis of diverse cytotoxic conditions such as cancer, various cardiovascular disorders and neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS), Parkinson’s disease (PD) and Alzheimer’s Disease (AD). The detection and quantification of superoxide within cells is of critical importance to understand biological roles of superoxide and to develop preventive strategies against free radical-mediated diseases.

Cyclic nitrone spin traps such as DMPO, EMPO, DEPMPO, BMPO and their derivatives have been widely used in conjunction with ESR spectroscopy for the detection of cellular superoxide with some success. However, the formation of unstable superoxide adducts from the reaction of cyclic nitrones with superoxide is a stumbling block in detecting superoxide by using Electron Spin Resonance (ESR).

The compounds of this invention can trap superoxide efficiently yielding UV-Visible products and even potentially fluorescence-detectable orange products. Hence, the chromotropic detection of superoxide using these compounds provides an improved method for free superoxide radical detection.

We are looking for a commercial partner to further develop these compounds as potential reagents for colorimetric assays for free superoxide radical detection.

APPLICATIONS

Method for superoxide radical detection.

ADVANTAGES
  • Easy to use colorimetric detection of superoxide in biological systems.
  • Reduced incidence of false negatives as compared to known chemiluminescent and fluorogenic radical detection probes.
  • Established method of production of the spin trapping agents.
CONTACT

For additional information about this technology opportunity, please contact Elizabeth Garami at egarami@fiu.edu or by phone at 305-348-0008 and ask about record IP 1101.