Novel Tools for DNA Topology and Topoisomerases Research

A new method for high throughput drug screening and DNA study.

TECHNOLOGY OPPORTUNITY

Florida International University (FIU) is pursuing a business partner interested in developing and commercializing Novel Reagents for the Study of DNA Topology and Topoisomerases. These tools are also useful for screening Anti-Cancer Drugs and Antibiotic Targeting DNA topoisomerases. FIU inventors have created a method to mass-produce a type of fluorescently labeled circular DNA molecules with high yields to study DNA topology and topoisomerases by fluorescence resonance energy transfer (FRET), and to screen anti-cancer drugs and antibiotics targeting DNA topoisomerases.

In a typical assay to study DNA supercoiling and topoisomerases, agarose gel electrophoresis is usually used to resolve DNA topoisomers produced in the reaction mixtures. Since gel electrophoresis is time-consuming it is desirable to develop other assays, such as fluorescence-based assays, to study DNA topology and DNA topoisomerases. The novel fluorescently labeled circular DNA molecules provide a quick and low-cost method to study DNA topology and topoisomerases through FRET.

We are looking for a business partner to further develop and commercialize these reagents as a viable alternative to commercially available DNA research techniques and to exploit its potential as a better method for screening anti-cancer drugs and antibiotics targeting DNA topoisomerases.

APPLICATION

The immediate and future applications of this technology are to provide a type of novel reagents to study DNA topology and topoisomerases by FRET and a new method for high throughput drug screening against DNA topoisomerases for anticancer drug discovery and antibiotics discovery.

ADVANTAGES
  • Provides tools to study DNA topology and topoisomerases in real time by FRET.
  • Facilitate the development of kits and reagents for high throughput screening (HTS) against DNA topoisomerases for anticancer drug and antibiotics discovery using FRET.
  • Allows for the production of fluorescence-labeled DNA molecules at milligram range.
  • Provides high quality of relaxed and supercoiled DNA molecules.
CONTACT

For additional information about this technology opportunity, please contact Elizabeth Garami at egarami@fiu.edu or by phone at 305-348-0008 and ask about record IP 1540.