Florida International University (FIU) is seeking a business partner to develop and commercialize Methods for Identifying Bivalent Reaction Mechanisms and Surface Plasmon Resonance (SPR) Sensorgrams that fit a bivalent analyte model.
Surface plasmon resonance (SPR) is an affinity based, label-free biophysical technique for the analysis of biomolecular interactions, including the determination of the kinetic parameters. To enable a better understanding of the interactions taking place, better predictions and analysis, as well as reduced computing resources, it is crucial to obtain accurate models and rate constants. Several attempts have been reported in order to enhance the resolution of SPR systems. However, there is no clear and straightforward procedure to choose and analyze the appropriate biphasic SPR data.
FIU inventors have developed new methods of analyzing the biphasic SPR data using the three commonly employed biphasic models. The methods are based on a general analytical solution of the biphasic rate equations, which is much more transparent and straightforward than the highly non-linear numerical integration approach. This method can be used to determine the underlying biphasic interaction mechanism from the analysis of the SPR data, and extract the rate constants with high confidence levels.
- Drug discovery
- Food and beverage quality and safety
- Life science
- Electrochemical analysis
- Environmental science
- Chemical sensor development
- Improve SPR analysis
- Obtain more accurate models and rate constants
- Reduce computing resources
- Provide better predictions of biomolecular interactions
- Can be adapted to existing data fitting software package